SFE-GC-MS coupling

Gas chromatography-mass spectrometry (GC-MS) combines the advantages of gas chromatography and mass spectrometry. It has strong separation ability, unique identification ability for unknown compounds, and extremely high sensitivity. The gas chromatography-mass spectrometry composed of gas chromatography and mass spectrometry can enhance the analytical ability of SFE extracts. Supercritical carbon dioxide extraction and gas chromatography-mass spectrometry were used to extract and detect four compounds in vanilla pods, namely vanillin, p-hydroxybenzaldehyde, vanillic acid, and p-hydroxybenzoic acid. The optimal extraction conditions were obtained as extraction temperature 50 ℃, extraction pressure 20 MPa, modifier ratio 10%, and static and dynamic cycle extraction 2 times. 50 chemical components were identified from the supercritical carbon dioxide extract of American cockroach by GC-MS, and the relative percentage content of each component was calculated by area normalization method. Among them, unsaturated fatty acids and esters accounted for 76.33% of the total extract content, which was the main component.